They developed a rapid method to isolate and identify heterogeneous CTCs with high efficiency from patient blood samples using the fluorescent-magnetic nanoparticles (FMNPs).
A dual-antibody interface targeting EpCAM and Ncadherin was fabricated onto the F-MNPs to capture epithelial CTCs as well as mesenchymal CTCs from whole blood samples.
Meanwhile, the F-MNPs provided a rapid identifying strategy for heterogeneous CTCs (F-MNPs circle+, Hoechst 33342+, and CD45") that could directly identify CTCs in a gentle one-step processing within 1 h after isolation.
Finally, the F-MNPs were validated in CTC isolation and CTC identification in the blood samples of cancer patients. (ÀÌÇÏ »ý·«)
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