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³í¹®¹ßÇ¥ PPT_High-Efficiency isolation and rapid identification of heterogeneous circulating tumor cells using dual antibody modified fluorescent magnetic nanoparticles ÆÄ¿öÆ÷ÀÎÆ® ÀÚ·áÀÔ´Ï´Ù
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High-Efficiency isolation and rapid identification of heterogeneous circulating tumor cells using dual antibody modified fluorescent magnetic nanoparticles, ACS Applied materials&interfaces_2019
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Introduction
Result
Discussion
º»¹®³»¿ë
They developed a rapid method
to isolate and identify heterogeneous CTCs with high efficiency from patient blood samples using the fluorescent-magnetic nanoparticles (FMNPs).
A dual-antibody interface targ
eting EpCAM and Ncadherin was fabricated onto the F-MNPs to capture epithelial CTCs as well as mesenchymal CTCs from whole blood samples.
Meanwhile, the F-MNPs provided
a rapid identifying strategy for heterogeneous CTCs (F-MNPs circle+, Hoechst 33342+, and CD45") that could directly identify CTCs in a gentle one-step processing within 1 h after isolation.
Finally, the F-MNPs were valid
ated in CTC isolation and CTC identification in the blood samples of cancer patients. (ÀÌÇÏ »ý·«)
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