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ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ

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TZ-SHR-1122299
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2020.08.15
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37 page / 2.20 MB
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ÀçÁ¶ÇÕ DNA¿Í ÇüÁúÀüȯ¿¡ ´ëÇØ ±â¼úÇÑ ¸®Æ÷Æ® Âü°íÀÚ·áÀÔ´Ï´Ù.

  • Microsoft PowerPoint (pptx)Microsoft PowerPoint (pptx)
DNAÇüÁúÀüȯÀüȯÇüÁúÀçÁ¶ÇÕ
¿¬°ü ÃßõÀÚ·á
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #1    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #2    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #3
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #4    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #5    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #6
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #7    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #8    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #9
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #10    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #11    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #12
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #13    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #14    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #15
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #16    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #17    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #18
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #19    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #20    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #21
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #22    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #23    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #24
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #25    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #26    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #27
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #28    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #29    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #30
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #31    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #32    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #33
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #34    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #35    ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #36
 ÀçÁ¶ÇÕDNA¿Í ÇüÁúÀüȯ #37        

  ÀçÁ¶ÇÕ dna (recombinant dna)   ÀçÁ¶ÇÕ dna  recombinant dna  hybrid dna  chemeric dna (chimera)  ÇüÁúÀüȯ (transformation)  
ÇüÁúÀüȯ, Á¢ÇÕ (conjugation), ÇüÁúµµÀÔ (transduction)
ÇüÁúÀüȯ : ÀçÁ¶ÇÕ dna¸¦ ¼÷Á¶ ¼¼Æ÷¿¡ µµÀÔ, ¹ßÇö½ÃÅ°´Â °úÁ¤
transformant  transgenic animal, transgenic plant,   gmo (genetically modified organism)  gmc (genetically modified crop)  lmo (living modified organism)  gem (genetically engineered microorganism)    
plasmid vector¸¦ ÀÌ¿ëÇÑ ÀçÁ¶ÇÕ dna ºÐÀÚÀÇ Á¦ÀÛ
for efficient ligation : Á¦ÇÑÈ¿¼ÒÀÇ ¼±ÅÃÀÌ °¡Àå Áß¿ä
dna fragment ¿Í vector¿¡ sticky end »ý¼º  
À¯ÀüÀÚ¸¦ Æ÷ÇÔÇÑ dna¿Í ¿î¹Ýü dna¸¦ °°Àº Á¦ÇÑÈ¿¼Ò·Î ó¸®
°°Àº sticky ends¸¦ »ý¼ºÇÏ´Â ´Ù¸¥ Á¦ÇÑÈ¿¼Ò·Î ó¸®
  ligation of sticky ends  ÀÚ°¡ ¿¬°á (self ligation)  ÀÚ°¡ ¿¬°á ¹æÁö (prevention of self-ligation)  vectorÀÇ Å»ÀλêÈ­ (dephosphorylation)   
Á¦ÇÑÈ¿¼Ò·Î Àý´ÜµÈ vector dnaÀÇ ÇÑ ÂÊ ¸»´ÜÀ¸·ÎºÎÅÍ 5¡¯-po4 Á¦°Å
ligation : 3¡¯-oh 5¡¯-po4   dephosphorylation of 5¡¯-po4 ¡æ 5¡¯-oh   3¡¯-oh 5¡¯-oh ¡æ no ligation  
alkaline phosphatase (cip, calf intestinal phosphatase)
  
º¤ÅÍÀÇ ÀÚ°¡ ¿¬°áÀ» ¸·±â À§ÇÑ Å»ÀλêÈ­ È¿¼ÒÀÇ Ã³¸®
  ligation of dna fragments with blunt ends  blunt end ¡æ sticky end  linker (¿¬°áÀÚ)  adaptor  homopolymer tailing   dna topoisomerase¸¦ ÀÌ¿ëÇÑ blunt end ¿¬°á    linker   
synthetic oligonucleotide (dsdna) : xxx-xxx bp
recognition sites for xxx-xxx restriction enzymes
ligated to blunt end dna by t4 dna ligase   efficient ligation at high concentration  
generation of cohesive ends : digestion with re
problem : the sites for the enzyme used to generate
cohesive ends may be present in the target dna fragment.
  
creation of cohesive ends on blunt-ended dna fragments.
suitable linkers are liga  
ted to the blunt ends by t4 dna ligase.the linker is then cleaved with the appropriate restriction enzyme to generate sticky ends.
  adaptor  linker with cohesive ends   linker digested with re   
one blunt end : 5¡¯-po4 one sticky end : 5¡¯-oh
3¡¯-oh 3¡¯-oh  no self-ligation :   
t4 dna ligase to ligate adaptors to the blunt-ended dna   (ÀÌÇÏ »ý·«)

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